Journal: iScience

Article Title: Agent-based modeling of neuronal mitochondrial dynamics using intrinsic variables of individual mitochondria

doi: 10.1016/j.isci.2025.112390

Figure Lengend Snippet: Neuronal recordings of mitochondrial dynamics (A) Representative image of MitoTracker Deep Red-labeled neurons from live cell recordings. Scale bar: 10 μm. (B) Representative frames 60 s apart of MitoTracker Deep Red-labeled mitochondria performing fusion. Scale bar: 1 μm. (C) Quantification of MitoTracker Deep Red signal over time in neuron recordings. (D) Quantification of mitochondria undergoing fusion and fission events, as percentage of total mitochondrial objects, over time in MitoTracker Deep Red recordings. (E) Representative image of MitoTimer neuron from live cell recordings. Scale bar: 10 μm. (F) Representative frames 120 s apart of MitoTimer expression mitochondria performing fission. Scale bar: 1 μm. (G) Quantification of MitoTimer green signal, red signal (left y axis), and red/green ratio (right y axis) over time in MitoTimer neuron recordings. (H) Quantification of mitochondria undergoing fusion and fission events, as percentage of total mitochondrial objects, over time in MitoTimer recordings. n = 4–5 biological replicates.

Article Snippet: For MitoTracker Deep Red recordings, cells were washed once with sterile PBS and incubated with 30nM MitoTracker Deep Red FM (Fisher Scientific, M22426 ) in Earle’s Balanced Salts Solution (EBSS) without phenol red (Fisher Scientific, AAJ67559AP) during 30 minute acclimation.

Techniques: Labeling, Expressing

Journal: iScience

Article Title: Agent-based modeling of neuronal mitochondrial dynamics using intrinsic variables of individual mitochondria

doi: 10.1016/j.isci.2025.112390

Figure Lengend Snippet: Dynamic mitochondria have unique intrinsic properties (A) Histogram of log-transformed mitochondrial area (μm 2 ) from individual mitochondrial objects during MTDR recordings. Curves are fitted Gaussian distributions. (B) Histogram of log-transformed aspect ratio (AR) from individual mitochondrial objects during MTDR recordings. Curves are fitted Gaussian distributions. (C) Histogram of log-transformed relative MitoTracker Deep Red (MTDR) mean intensity from individual mitochondrial objects during MTDR recordings. Curves are fitted Gaussian distributions. (D) Correlation matrix of intrinsic variables collected from individual mitochondrial objects during MTDR recordings. (E) Histogram of log-transformed MitoTimer red/green ratio from individual mitochondrial objects during MitoTimer recordings. Curves are sum of two Gaussian distributions. (F) Correlation matrix of intrinsic variables collected from individual mitochondrial objects during MitoTimer recordings.

Article Snippet: For MitoTracker Deep Red recordings, cells were washed once with sterile PBS and incubated with 30nM MitoTracker Deep Red FM (Fisher Scientific, M22426 ) in Earle’s Balanced Salts Solution (EBSS) without phenol red (Fisher Scientific, AAJ67559AP) during 30 minute acclimation.

Techniques: Transformation Assay

Journal: iScience

Article Title: Agent-based modeling of neuronal mitochondrial dynamics using intrinsic variables of individual mitochondria

doi: 10.1016/j.isci.2025.112390

Figure Lengend Snippet: Intrinsic mitochondrial properties predict fission and fusion events (A) ROC curve of multiple logistic regression classifying stable and fission mitochondria from MTDR recordings. (B) Violin plot of predicted probability for fission of MTDR-labeled mitochondria by multiple logistic regression. (C) ROC curve of multiple logistic regression classifying stable and fusion mitochondria from MTDR recordings. (D) Violin plot of predicted probability for fusion of MTDR-labeled mitochondria by multiple logistic regression. (E) ROC curve of multiple logistic regression classifying stable and fission mitochondria from MitoTimer recordings. (F) Violin plot of predicted probability for fission of MitoTimer mitochondria by multiple logistic regression. (G) ROC curve of multiple logistic regression classifying stable and fusion mitochondria from MitoTimer recordings. (H) Violin plot of predicted probability for fusion of MitoTimer mitochondria by multiple logistic regression.

Article Snippet: For MitoTracker Deep Red recordings, cells were washed once with sterile PBS and incubated with 30nM MitoTracker Deep Red FM (Fisher Scientific, M22426 ) in Earle’s Balanced Salts Solution (EBSS) without phenol red (Fisher Scientific, AAJ67559AP) during 30 minute acclimation.

Techniques: Labeling